Proposal

Narrative

This proposal seeks funding for a fluorescence microscope and image analysis software for the ESHP biochemistry lab that currently has no microscopy capability. I am requesting funding for the Keyence microscope because as a self-contained and fully-automated system 1) I, a quadruple amputee, can use it relatively independently; 2) it is a less daunting introduction to microscopy for the novice student; and 3) it will increase throughput and thus research productivity. For e.g., in a single experiment, I typically engage 4-5 undergraduate students, each of whom takes responsibility for a protein-quantification project which can culminate in honor’s theses, posters, or co-authorship on a publication. Protein quantification takes several months. Easy access to a scope to use as schedules permit, the fast image acquisition/stitching capability of the Keyence scope coupled with the automated target detection/quantification and capability of the software will greatly accelerate this process. The software will also increase accessibility as its use is not tied to a physical location. Importantly, the software uses AI technology which is in concert with FAU’s designation of “University Distinction” for AI and aligns with the CoS goals. It will serve as an exciting and insightful introduction to machine learning and its application. The microscope will be available to all interested FAU faculty. It will supplement instruction at the graduate and undergraduate level in ESHP classes such as Neurophysiology of Human Movement (PET 3050), Human Systems Physiology in Exercise Science (PET 6356), and Skeletal Muscle Plasticity (PET 6382). The acquisition of the microscope and AI-powered software will 1) facilitate our department’s research and teaching capabilities; 2) promote student scholarship and success; and 3) increase accessibility to basic science techniques. As such, it will significantly enhance our department’s contributions to the advancement of FAU’s strategic plan.

Facilities

N/A

Hardware Requirements

All-In-One BZ-X800E Keyence microscope and computer included in budget.

Software Requirements

5-yr license for PIPSQUEAK AI plugin included in budget to be used with freely available NIH ImageJ software.

Personnel Costs

N/A

Other Costs

N/A

Timeline

With acquisition of microscope and software in Spring 2022 and tissue already available, research use is expected to begin immediately and instructional use will begin in Summer 2022.

Sustainability

Hardware repair, if needed, beyond its warranty date and software license renewal will be paid for by departmental, internal, or extramural funds.

Resource Matching

N/A

Implementing Organization

College of Science, Exercise and Health Promotion Department.